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Avantor nerve growth factor (ngf) elisa ngf rapid elisa kit: mouse
In vitro cAMP assay (A), <t>NGF</t> <t>ELISA</t> (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.
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In vitro cAMP assay (A), <t>NGF</t> <t>ELISA</t> (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.
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In vitro cAMP assay (A), <t>NGF</t> <t>ELISA</t> (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.
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Proteintech human ngf elisa kit
In vitro cAMP assay (A), <t>NGF</t> <t>ELISA</t> (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.
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USCN Life elisa kits specific for mouse ngf
In vitro cAMP assay (A), <t>NGF</t> <t>ELISA</t> (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.
Elisa Kits Specific For Mouse Ngf, supplied by USCN Life, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro cAMP assay (A), NGF ELISA (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.

Journal: Journal of Bone and Mineral Research

Article Title: Pain in fibrous dysplasia: identifying nociceptive mechanisms in a preclinical model

doi: 10.1093/jbmr/zjaf039

Figure Lengend Snippet: In vitro cAMP assay (A), NGF ELISA (B), and multiplex assay (C-K). FD cells and control cells were activated with doxycycline (10 μg/mL) or vehicle (phosphate buffered saline) for 24 hr with IBMX. Intracellular cAMP was assessed using cAMP Gs dynamic kit and protein factors were assessed using MSD U-PLEX assay. Two-way ANOVA with Tukey’s multiple comparison test. Graphs are individual values with bars representing median ± IQR. **** p < .0001. Abbreviations: cAMP, cyclic adenosine monophosphate; FD, fibrous dysplasia; IBMX, 3-isobutyl-1-methylxanthine; NGF, nerve growth factor.

Article Snippet: cAMP concentration assay (Cisbio GS Dynamic Kit, PerkinElmer), Meso Scale Discovery Multiplex assay (U-PLEX; MSD), and nerve growth factor (NGF) ELISA (NGF Rapid ELISA kit: mouse; Avantor) were conducted according to manufacturer directions.

Techniques: In Vitro, cAMP Assay, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Control, Saline, Plex Assay, Comparison